Venom Protein Card
Accession: Pre01673 Bicarinaline [Tetramorium bicarinatum]
Basic info
| Organism | Tetramorium bicarinatum |
| Taxonomy | Insecta > Hymenoptera > Formicidae > Tetramorium > Tetramorium bicarinatum |
| Protein Description | Bicarinaline |
| Mass Weight | 8171.57 Da |
| Isoelectric Point | 5.59 |
| Expression | Highly expressed in venom gland |
Annotation
| Function | Antimicrobial peptide that shows antimicrobial activities against all microorganisms tested with minimal inhibitory concentrations (MICs) values ranging from 0.45 to 97.5 umol/L. This peptide kills the microorganisms by permeabilizating the membranes. It shows a very weak hemolytic activity (HC(50)=325 umol/L) and weak cytotoxicity against human lymphocytes (LC(50)=67.8 umol/L). Gram-negative bacteria tested are E.coli (MIC=24.4 umol/L), C.sakazakii (MIC=5.8 umol/L), P.aeruginosa (MIC=8.7-12.2 umol/L), S.enterica (MIC=5.4 umol/L), and H.pylori (MIC=0.99-3.9 umol/L). Gram-positive bacteria tested are E.hirae (MIC=12.2 umol/L), S.aureus (MIC=3.0-6.4 umol/L), methicillin-resistant S.aureus (MRSA) (MIC=8.7 umol/L), S.xylosus (MIC=0.45-1.3 umol/L), and B.subtilis (MIC=24.4 umol/L). Fungi tested are A.niger (MIC=0.75 umol/L), C.albicans (MIC=17.3 umol/L), G.candidum (MIC=97.5 umol/L), and S.cerevisiae (MIC=6.1 umol/L). Finally the parasite tested is L.infantum (MIC=1.5 umol/L). | ||
| Pfam |
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Features
| Feature key | Position | Length |
| Signal Peptide | 1..26 | 26 |
| Propeptide | 27..56 | 30 |
| Peptide | 57..76 | 20 |
Sequence
Publication
| 1. |
Tene N., Bonnafe E., Berger F., Rifflet A., Guilhaudis L., Segalas-Milazzo I., Pipy B., Coste A., Leprince J., Treilhou M.; "Biochemical and biophysical combined study of bicarinalin, an ant venom antimicrobial peptide."; Peptides 79:103-113(2016). |
| 2. |
Bouzid W., Klopp C., Verdenaud M., Ducancel F., Vetillard A.; "Profiling the venom gland transcriptome of Tetramorium bicarinatum (Hymenoptera: Formicidae): the first transcriptome analysis of an ant species."; Toxicon 70:70-81(2013). |
| 3. |
Rifflet A., Gavalda S., Tene N., Orivel J., Leprince J., Guilhaudis L., Genin E., Vetillard A., Treilhou M.; "Identification and characterization of a novel antimicrobial peptide from the venom of the ant Tetramorium bicarinatum."; Peptides 38:363-370(2012). |
| 4. |
Guzman J., Tene N., Touchard A., Castillo D., Belkhelfa H., Haddioui-Hbabi L., Treilhou M., Sauvain M.; "Anti-helicobacter pylori properties of the ant-venom peptide bicarinalin."; Toxins 10:1-10(2017). |
| 5. |
Touchard A., Aili S.R., Fox E.G., Escoubas P., Orivel J., Nicholson G.M., Dejean A.; "The biochemical toxin arsenal from ant venoms."; Toxins 8:1-28(2016). |