Venom Protein Card
Accession: Pre05206 Phospholipase A2 [Apis mellifera]
Basic info
| Organism | Apis mellifera |
| Taxonomy | Insecta > Hymenoptera > Apidae > Apis > Apis mellifera |
| Protein Description | Phospholipase A2 |
| Mass Weight | 18486.69 Da |
| Isoelectric Point | 7.18 |
| Expression | Highly expressed in venom gland |
Annotation
| Function | PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.Three segments were taken from the known X-ray structure of bovine pancre- atic phospholipase A2 [6] which form the active site and the region which binds the Ca2' ion. | ||
| Pfam |
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Features
| Feature key | Position | Length |
| Signal Peptide | 1..18 | 18 |
| Propeptide | 19..33 | 15 |
| Chain | 34..167 | 134 |
Sequence
Publication
| 1. |
Moreira L.A., Ito J., Ghosh A., Devenport M., Zieler H., Abraham E.G., Crisanti A., Nolan T., Catteruccia F., Jacobs-Lorena M.; "Bee venom phospholipase inhibits malaria parasite development in transgenic mosquitoes."; J. Biol. Chem. 277:40839-40843(2002). |
| 2. |
Valdez-Cruz N.A., Segovia L., Corona M., Possani L.D.; "Sequence analysis and phylogenetic relationship of genes encoding heterodimeric phospholipases A2 from the venom of the scorpion Anuroctonus phaiodactylus."; Gene 396:149-158(2007). |
| 3. |
Kuchler K., Gmachl M., Sippl M.J., Kreil G.; "Analysis of the cDNA for phospholipase A2 from honeybee venom glands. The deduced amino acid sequence reveals homology to the corresponding vertebrate enzymes."; Eur. J. Biochem. 184:249-254(1989). |
| 4. |
Shipolini R.A., Callewaert G.L., Cottrell R.C., Vernon C.A.; "The amino-acid sequence and carbohydrate content of phospholipase A2 from bee venom."; Eur. J. Biochem. 48:465-476(1974). |
| 5. |
Ferreira Junior R.S., Sciani J.M., Marques-Porto R., Junior A.L., Orsi R.D., Barraviera B., Pimenta D.C.; "Africanized honey bee (Apis mellifera) venom profiling: Seasonal variation of melittin and phospholipase A(2) levels."; Toxicon 56:355-362(2010). |
| 6. |
Shipolini R.A., Doonan S., Vernon C.A.; "The disulphide bridges of phospholipase A2 from bee venom."; Eur. J. Biochem. 48:477-483(1974). |
| 7. |
Kubelka V., Altmann F., Staudacher E., Tretter V., Marz L., Hard K., Kamerling J.P., Vliegenthart J.F.; "Primary structures of the N-linked carbohydrate chains from honeybee venom phospholipase A2."; Eur. J. Biochem. 213:1193-1204(1993). |
| 8. |
Scott D.L., Otwinowski Z., Gelb M.H., Sigler P.B.; "Crystal structure of bee-venom phospholipase A2 in a complex with a transition-state analogue."; Science 250:1563-1566(1990). |